不同光照条件对槲蕨组织培养外植体褐化的影响

Effect of different illumination on browning of Drynaria fortunei

采用组织培养方法,探讨了黑暗、1 000 lx、2 000 lx和3 000 lx等4种不同光照条件对槲蕨外植体褐变程度的影响。结果表明:随着光照时间的延长,槲蕨外植体的PPO、POD和总酚含量均表现为先增加后减少的趋势;在相同培养时间下,随着光照强度的增加,槲蕨外植体的PPO和POD活性也同时增加,其中光照强度1 000 lx处理的PPO和POD的活性均低于其他光照强度处理;光照对槲蕨外植体的总酚含量在相同培养时间下影响并不直接,但1 000 lx处理下的槲蕨外植体的总酚含量积累较少,说明1 000 lx是槲蕨的组织培养较为适宜的光照条件。

With the method of tissue culture, studies on the change law of explant browning at the different treatment of light（dark, 1 000 lx, 2 000 lx and 3 000 lx）. The result showed that the PPO, POD and total phenols content increased first and decreased later during the light time. At the same culture time, with the increase of light intensity, the activity of PPO and POD increasing, and the value of POD of 1 000 lx treatment were smaller than other light. The total phenols content did not display ,. directly to luminance. However, like PPO and POD, the content of 1 000 lx treatment also showed smallest. Therefore, the appropriate condition in the tissue culture of Drynaria firtunei was the light of 1 000 lx.