广东甘蔗宿根矮化病菌的分离培养及鉴定

Isolation and identification of Leifsonia xyli subsp.xyli, the causal agent of sugarcane ratoon stunting disease from Guangdong cane area

利用改良的SC培养基,从甘蔗蔗汁中分离培养到一种杆状细菌,根据菌落的形态特征,并结合血清学和PCR检测方法,确认所分离到的菌是甘蔗宿根矮化病的致病菌Leifsonia xyli subsp.xyli。该菌的分离培养在广东还是首次报道,这使分离高纯度的甘蔗宿根矮化病菌DNA成为可能,促进了该菌以DNA为基础的检测方法的发展,同时也为今后对该菌开展遗传多样性等研究奠定了基础。

A tiny-colony bacilliform bacterium was isolated from sugarcane juice, using modified SC medium. According to the colony＇s characteristic and the results of serological and PCR-based diagnostic tests, the bacterium was confirmed as Leifsonia xyli subsp, xyli, the causal agent of sugarcane ratoon stunting disease（RSD）. The successful isolation of RSD bacterium, will of course, facilitate the research for the genetic diversity and preparation of RSD antibody in the future.