石家庄市5岁以下儿童轮状病毒感染状况研究

Study about rotavirus infection among infants within 5 years in Shijiazhuang

目的：掌握石家庄市5岁以下腹泻儿童轮状病毒的感染状况。方法：于2006年11月～2007年11月在河北省儿童医院和石家庄市第一医院收集5岁以下儿童疑似病毒性腹泻病例粪便标本,用酶联免疫吸附试验（ELISA）法检测标本中HRV抗原,对部分阳性标本采用反转录-聚合酶链反应（RT-PCR）进行HRVG血清型和P基因型分型。结果：170份标本HRV抗原107份阳性,阳性率62.9%。G血清型以G3型为主,未发现G4血清型。P血清型以P8为主,未发现P6、P9、P10等基因型。GP血清组合除常见的G1P[8]、G2P[4]、G3P[8]外,出现G9P[8]组合。结论：HRV是石家庄市婴幼儿腹泻的重要病原,流行的主要血清型为G3型。

Objective： To investigate the distribution and characteristics of rotavirus infections within 5 years in Shijiazhuang.Methods：The stool specimens were collected from patients with diarrhea within 5 years,from Hebei Provincial Children Hospital and Shijiazhuang 1st Hospital,from November 2006 to November 2007.The stool specimens were detected for rotavirus antigen with Enzyme-linked immunoadsorbt assay（ELISA）.Reverse transcription poltmerase chain reaction（RT-PCR） was performed to identify rotavirus G serotypes and P serotypes of antigen positive samples.Results：Among 170 specimens,the RV antigen positive rate was 62.9%.Serotype G3 and P8 was the dominant type.No G4 and P6,P9,P10 serotype.Besides G1P[8],G2P[4],G3P[8],we found G9P[8].Conclusion：RV is the dominant etiological agent for infantile within 5 years in Shijiazhuang and the predominant serotype is G3.