3种梅毒筛查方法的比较

Comparison of three screen methods for syphilis

目的:选择高敏感度、高特异度的梅毒血清学筛查方法,提高检出率,为卫生检疫执法提供技术支持。方法:分别用TP-ELISA、TRUST、金标法对102份TPPA确证阳性血清,5份TPPA可疑血清,220份TPPA确证阴性血清进行实验,比较3种梅毒初筛方法的敏感度和特异度。结果:TP-ELISA敏感度98.04%,特异度100%;TRUST敏感度78.43%,特异度99.55%;快速法敏感度72.55%,特异度100%。结论:作者认为对出入境人员的梅毒监测可采用TP-ELISA作为筛查方法,加急标本使用快速法。阳性用TPPA进一步确证。确证阳性做TRUST。根据滴度的变化判断梅毒的治疗效果及传染性,为检疫执法提供技术支持。

Objective： To select screening method for syphilis with satisfactory sensitivity and specificity.Methods：Applying TP-ELISA,TRUST and colloidal gold test to test 102 positive sera,220 negative sera and 5 indeterminable sera were tested by TPPA,respectively.Then we compared the sensitivity and specificity of the three methods.Results：The sensitivities of TP-ELISA,TRUST and IGLT were 98.04%,78.43% and 72.55%,respectively.Their specificities were 100%,99.55% and 100%,respectively.Conclusion：We suggest that the laboratory of quarantine system adopt TP-ELISA as the routine screen method for syphilis.Colloidal gold test could be used in emergency situation.TPPA could be used for confirming possible positive specimen.TRUST could be used to estimate the therapeutic effectiveness and infectivity of syphilis.