胆道闭锁血清蛋白质标记物的检测

Serum protein markers for biliary atresia

目的寻找特异性诊断胆道闭锁的血清蛋白标记物。方法应用表面增强激光解吸电离飞行时间质谱（SELDI—TOF-MS）技术检测28例血清标本（胆道闭锁7例，先天性胆总管囊肿7例，婴肝综合征3例，正常对照11例）的蛋白质质谱表达，结合生物信息学方法（SVM）分析数据。结果筛选出m／z位于3403、2108、2111、2131、2823的5个蛋白质标记物区分肝胆疾病和正常对照小儿的血清蛋白指纹图谱模型，敏感性94．1％，特异性81．8％；筛选出m／z位于3403蛋白质标记物区分胆道闭锁和正常对照小儿的血清蛋白指纹图谱模型，敏感性100％，特异性100％；筛选出m／z位于3403、4796的2个蛋白质标记物区分胆道闭锁和其他肝胆疾病的血清蛋白指纹图谱模型，敏感性71．4%，特异性80．0％。结论应用SELDI-TOF-MS结合SVM构建的胆道闭锁血清蛋白指纹图谱模型为胆道闭锁的早期诊断方法开拓了新方向，值得进一步研究。

Objective To select the specific serum protein markers for early diagnosis of biliary atresia （BA）. Methods The protein mass spectrograms were detected by the surface-enhanced laser desorption/ionization time of flight mass spectrometry （SELDI TOF-MS） technology combining with bio-informatics methods （support vector machine） in 28 serum samples （7 from BA, 7 from choledochal cyst, 3 from infant hepatitis syndrome, 11 from normal controls）. Results Five protein mark ers which m/z located at 3 403, 2 108, 2 111, 2 131, and 2 823 were selected for separation of hepatic and biliary diseases from normal controls with sensitivity of 94. 1%0 and specificity of 81.8%. The protein marker which m/z located at 3 403 was selected for separation of BA from normal controls with sensitivity of 100% and specificity of 100%. Two protein markers which m/z located at 3 403 and 4 796 were selected for separation of BA from infant hepatitis syndrome with sensitivity of 71.4% and specificity of 80.0%. Conclusions The fingerprint chromatogram of serum protein is a new method for early diagnosis of BA.