摘要
分别用 ras 和 myc 癌基因对一株 SV-40转化的肝细胞系进行了转化试验以及转化细胞的诱瘤性试验。我们的结论是:①1株 SV-40转化的肝细胞系——CWSV1细胞系能被 ras 和myc DNA 分别转化;②ras 和 myc DNA 的转染不影响 CWSV1细胞的分泌白蛋白的功能;③ras DNA的转染诱发了 CWSV1细胞的致瘤潜能。
The ability of the ras or myc DNA sequences to convert a low passage SV-40-immortalized hepato- cyte cell line into a tumorigenic cell line was tested.CWSV1 cells(p 19)were transfected with neo, neo+ras,and neo+myc.The neo and neo+myc transformants were not tumorigenic and secreted al- bumin and produced albumin RNA at the same levels as did the CWSV1 cells.The neo+ras transfor- ments were highly tumorigenic and continued to secrete albumin and produce albumin RAN at the same levels as did the CWSV1 cells.We conclude that at least one SV-40-immortalized hepatocyte cell line can be made tumorigenic by the introduction of ras DNA sequences and that this alteration does no diminish liverlike expression of albumin by this cells.
