固体培养条件对蛹虫草产子实体和虫草菌素的影响

Effects of Different Solid Culture Condition on Fruit Body and Cordycepin Output of Cordyceps militaris

研究了营养液不同pH值和米粒大小两个培养条件对蛹虫草固体发酵产子实体和虫草菌素的影响。当营养液pH值为5．5～6．0时子实体的产量最大，营养液pH值为6．5时，虫草菌素总的产量最大，达48．44g／瓶。较大的米粒有利于蛹虫草子实体的生长，较小的米粒有利于蛹虫草虫草菌素的积累。子实体的产量随着米粒的变小而减少；子实体中虫草菌素的含量随着米粒的变小而增加。当采用整米作为培养基基质时，蛹虫草子实体产量最大，达1．67g／瓶，当采用40目米＋10目米（1：1，M／M）作为培养基基质时，蛹虫草子实体中虫草菌素含量最大，达1．87％，虫草菌素总的产量也最大，达52．46mg／瓶。

The solid media with different pH value and rice grain size were used in the fermentation of Cordyceps militaris to study the effects on the fruit body and cordycepin output. The results show that the maximum fruit body yield and cordycepin output （48.44g/bottle） is obtained on the medium with pH 5.5-6.0 and pH 6.5 respectively, the fruit body yield reduces with decrease of rice grain size and the cordycepin content in the fruit body reduces with increase of rice grain size. The maximum fruit body yield （1. 67 g/bottle） is obtained on the medium with whole rice grains and the maximum cordycepin content in the fruit body （1.87 %） and cordycepin output （52.46mg/bottle） are obtained the medium with 40 and 10 mesh of rice grain size （1 ： 1,M/M）.