摘要
建立了OPA-FMOC柱前衍生化HPLC法同时测定复方氨基酸注射液中13种氨基酸和牛磺酸的含量。采用C18柱,梯度洗脱,自动在线衍生,检测波长338/262nm。结果表明13种氨基酸和牛磺酸均线性关系良好(r>0.9992),各组分的平均回收率为99.2%～101.5%,RSD为0.11%～0.92%(n=9)。
A HPLC method was established for the simultaneous determination of thirteen amino acids and taurine by HPLC with pre-column derivatization by OPA-FMOC, A C18 column was used with gradient elution and online derivatization at the detection wavelength of 338/262nm. All calibration curves of thirteen amino acids and taurine were linear （r〉0,9992）. The average recoveries were 99.2 % - 101.5 %, with RSDs of 0.11% - 0.92 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第8期610-612,共3页
Chinese Journal of Pharmaceuticals
关键词
午磺酸
氨基酸
高效液相色谱
柱前衍生
测定
taurine
amino acid
HPLC
pre-column derivatization
determination
