慢性乙型肝炎患者病程中CD5^+ B细胞及CD4/CD8的变化研究

Changes of CD5^+ B cell and CD4/CD8 T cell ratio in patients with chronic hepatitis B

目的研究CD5+B细胞和CD4/CD8在慢性乙型肝炎发病中的作用,以及他们之间的相关性。方法采用免疫荧光双标记技术和流式细胞仪对48例慢性乙型肝炎患者发病前后周围血中CD4/CD8比例和CD5+B细胞的百分率和37例健康对照者进行比较。结果在慢性乙型肝炎患者发病高峰期周围血CD4/CD8比例显著低于病情恢复期,且和健康对照组比较差异有显著性;而CD5+B细胞在慢性乙型肝炎患者发病高峰期,显著高于健康对照组和慢性乙型肝炎患者恢复期,差异有显著性。结论慢性乙型肝炎的发病和T细胞免疫功能的紊乱相关,而CD5+B可能在乙型肝炎的慢性化机制中发挥作用。

Objective To study the role of CD5 ^＋ B cells and the ratio of CD4/ CD8 in the pathogenic mechanism of chronic hepatitis B. Methods The percentages of CD5^ ＋ B cell （ CD5^＋ CD19^＋） and the ratio of CD4/CD8 in 48 patients with chronic hepatitis B and 37 healthy control were determined using immunofluorecence double - staining and flow cytometry. Results The CD4/CD8 ratio was significantly increased in chronic hepatitis B patients during hepatitis exacerbation compared with subsequent convalescence, and healthy controls. The percentages of CD5^ ＋ B cell was significantly increased in chronic hepatitis B patients during hepatitis exacerbation compared with that in healthy controls, but not in convalescence stage of the patients. Conclusion The development of hepatitis B was connected with the derangement of T lymphocytes closely, and CD5^＋ B cell may play a role in protracted course of disease.