运动对糖尿病大鼠认知功能的影响

The effect of exercise on cognitive function in diabetic rats

目的探讨运动对糖尿病大鼠认知功能的影响。方法将雌性SD大鼠30只随机分为3组:正常对照组(n=10)、糖尿病组(n=10)和糖尿病运动组(n=10)。采用链脲佐菌素(STZ)腹腔内注射建立糖尿病模型。糖尿病运动组大鼠每日游泳训练1次,共计运动3个月。分别在建模前和建模后3个月检测大鼠的血糖和体质量,Morris水迷宫测试大鼠学习记忆能力,免疫组织化学方法检测脑组织海马区纤维连接蛋白(Fn)分布情况。结果成模后,糖尿病组和糖尿病运动组大鼠血糖均高于正常对照组(P<0.01)。大鼠学习记忆能力,糖尿病组与正常对照组相比显著下降(P<0.01);糖尿病运动组明显好于糖尿病组(P<0.01)。海马区脑组织Fn阳性区域,糖尿病组比正常对照组显著增加(P<0.01);糖尿病运动组增加不明显(P>0.05)。结论运动可以改善糖尿病大鼠的学习记忆能力,并对糖尿病大鼠血-脑脊液屏障起保护作用。

Objective To study the effect of exercise on cognitive function and blood - brain barrier in diabetic rats. Methods Thirty female SD rates were randomly chosen and divided into three groups： normal control group （ NC, n =10）, diabetic group （ n = 10）, and diabetic exercise group （ n = 10）, The rat models of diabetes mellitus were established by intraperitioneal injection of streptozotocin. Diabetic exercise rats were additionally trained by swimming once every day for three months, The following indexes were measured before treatment and three months after the establishment of rat models, Blood glucose level and weight of diabetic rats were examined. The learning and memory function was evaluated by Morris maze, Fibronectin （FN） expression in hippocampas of rats was detected with immunohistochemical method. Results After all rat models were established, the level of blood glucose in rats in diabetic group and diabetic exercise group was significantly increased in comparison with that of rats in NC group （ P 〈 0. 01 ）, Compared with NC group, the learning and memory function in rats of diabetic group was significantly decreased （ P 〈0. 01 ）, and learning and memory function in rats of diabetic exercise group was remarkably increased in comparison with that of rats in diabetic group, Compared with NC group, the expression level of FN in hippocampus in rats of diabetic group was significantly increased （ P 〈 0.01 ）, but no significant difference was found in expression level of FN between rats in diabetic group and diabetic exercise group （ P 〉 0. 05 ）, Conclusion The results indicate that exercise can improve the learning and memory function of diabetic rats, and protect the blood - brain barrier of diabetic rats.