人小肠上皮细胞缺氧复氧损伤的预防研究

Studies on the Prevention of Anoxia/Reoxygenation Induced Injury of Human Intestinal Epithelial Cells in Vitro

应用原代培养人小肠上皮细胞（IEC），观察缺氧复氧（A／R）对体外培养人IEC的损伤作用。结果表明：A／R可使IEC细胞存活率下降和乳酸脱氨酶（LDH）漏出增加，二者呈显著的负相关，其变化程度与A／R时间有依从关系。在缺氧前或缺氧后复氧前联合应用超氧化物歧化酶和过氧化氢酶可减轻A／R诱发的细胞存活率下降和LDH漏出量增加，应用别嘌呤醇世有同样的保护作用。表明：人IEC在复氧时可产生超氧基并引起细胞损伤。

We studied the injurious effect of anoxia/reoxygenation (A/R) on human intestinal epithelial cells (IEC) in primary culture. The results showed that A/R could reduce the viability of human IEC and increase the leakage of lactate dehydrogenase (LDH) from the cells. They had a negative correlationship and the variations of both were dependent on the duration of A/R acting on the cells. This injury was reduced by treatment with superoxide dismutase and catalase together, either before anoxia or after anoxia, but just before reoxygenation. Similar protection could be provided by allopurinol. The above results suggested that A/R could 'injure human IEC; and the injury occurred primarily at reoxygenation and was mediated by a process depending upon superoxide formation.