受体细胞因子TGF-β1和TNF-α的基因多态性与肝移植急性生物学排斥的关系

The relationship between recipient cytokine (TNF-α and TGF-β1) genes polymorphism and acute biological rejection after liver transplantation

目的:研究受体肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的基因调节区或启动子区基因多态性分布与肝移植急性生物学排斥的关系。方法:取63例肝移植病人和60例正常人外周静脉血2mL,加入400μL5%EDTA抗凝,用QIAamp DNA Blood Midi Kit抽提DNA。应用美国One-Lambda公司提供的细胞因子基因型检测盒,采用序列特异性引物聚合酶链反应(PCR-SSP)法,行细胞因子基因调节区或启动子区多态性分析。结果:病人年龄、性别、Child分级、手术方式、肝脏原发疾病、血型不配对与急性生物学排斥无明显关系。TNF-α高表达型与低表达型的比例在排斥组中明显高于非排斥组(10/15vs5/33,P=0.016),排斥组中受体TNF-α高表达/受体TGF-β1高表达组合对其他表达形式的比例(9/16)显著高于非排斥组(4/34),P=0.015。结论:受体TNF-α高表达/受体TGF-β1高表达可能是急性生物学排斥反应的危险因素。

Objective To explore the relationship between recipient cytokine （TNF-α,TGF-β1） genes polymorphism and acute biological rejection after liver transplantation. Methods Peripheral venous blood samples added with 400μL 5% EDTA from 63 recipients and 60 normal persons were collected for DNA extraction using QIAamp DNA Blood Midi Kit. Cytokine gene polymorphism were determined using polymerase chain reaction sequence-specific primers （PCR-SSP） technique with commercially available kits （One Lambda Inc.,USA）. Results The age, gender, Child score, mode of operation, primary liver diseases and unmatched transplantation were not associated with acute biological rejection after liver transplantation. The ratio of high TNF-α profiles to low TNF-α profiles of recipient in the rejection group was significantly higher than that in the non-rejection group（10/15 vs 5/33, P=0.016）. The high TNF-α profiles/high TGF-β1 profiles were much more common in the rejection group than those in the non-rejection group （9/16 vs 4/34, P=0.015）. Conclusions The recipient high TNF-α profiles/high TGF-β1 profiles might be served as a risk factor of acute biological rejection after liver transplantation.