新疆维吾尔族与汉族子宫内膜异位症患者在位内膜相关基因表达的差异

Differential expressed genes in eutopic endometrium of endometriosis patients： comparison between Uygur and Han women in Xianjiang

目的从分子水平探讨子宫内膜异位症（EM）发病的民族差异性，从而为新疆维吾尔族妇女EM病因、诊断及治疗的研究提供科学的理论根据。方法选择维吾尔族EM患者10例，汉族EM患者16例，对照组22例（其中维吾尔族10例，汉族12例），取维吾尔族和汉族EM患者在位内膜RNA每份各取6μg混合分别作为实验组，非EM患者正常内膜RNA每份各取6μg混合作为对照组，运用Atlas^TM cDNA Expression Arrays（Clontech #7854-1）基因表达谱芯片（含有22000条Cdna），筛选出3次实验上下调趋势一致的共同差异基因。结果芯片结果分析，维吾尔族EM组获得共同显著差异基因11个，其中上调5个，下调6个；汉族EM组获得共同显著差异基因58个，其中上调53个，下调5个；维吾尔族EM组和汉族EM组共同差异基因5个，2个下调，3个上调。差异基因筛选标准：实验的M值的绝对值〉1，并且Flag=0或Flag=100。结论新疆地区汉族和维吾尔族子宫内膜异位症患者的发病存在民族差异性，维吾尔族内异症在位内膜的差异基因相对汉族少，可能是维吾尔族妇女内异症发病率相对较低的遗传学原因。

Objective To investigate the reason why the incidence of endometriosis （EM） is lower in the Uygur women than in the Han women. Methods Eutopic and ectopic endometrium samples were obtained by operation and biopsy from 26 EM patients, 10 Uygur women and 16 Han women and analyzed with a gene expression microarray containing the cDNAs of 22 000 human genes. Twenty-two women, 10 Uygur and 12 Han, were used as controls. Results Eleven differentially expressed genes, 7 up-regulated and 6 down-regulated, were screened out from the eutopic endometrium of the Uygur women with EM. 58 differential expressed genes were screened out from the in eutopic endometrium of the Han women with EM, 53 being up-regulated and 5 down-regulated. Five genes were screened out in both groups, 3 being up- regulated and 2 down-regulated. Conclusion The number of differentially expressed genes of the Uygur women with EM is lower than that of the Han women with EM, which may be the cause of relative low incidence of EM among Uygur women.