营养水平对PRKAG3基因表达量及对肉质影响的研究

Effect of Nutritional Level on PRKAG3 Gene Expression and Pork Quality

挑选70 kg左右的DLY猪12头,随机分为2组,分别饲喂高、低2种水平的日粮,高营养水平为DE 13.81MJ/kg、CP 14%,低营养水平为DE 12.55 MJ/kg、CP 11%。体重达到100 kg左右时屠宰,测定胴体性状、肉质性状和PRKAG3基因表达量。以探讨营养水平对PRKAG3基因表达量及肉质的影响。结果表明:低营养水平有促进PRKAG3基因表达的趋势(P>0.05);高营养水平有提高屠宰率、瘦肉量、瘦肉率、眼肌面积、L值、a值和b值的趋势(P>0.05),但滴水损失显著低于低营养水平(P<0.05);营养水平对PRKAG3基因表达量有一定的影响。猪PRKAG3基因表达量与瘦肉率、眼肌面积、a值、b值和滴水损失呈正相关,但相关性均不显著(P>0.05);与pH2呈显著负相关(P<0.05)。这表明营养水平对PRKAG3基因表达量有一定的影响,进而可影响肉质。

12 DLY pigs（70 kg） were randomly divided into two groups with diets of high （DE 13.81 MJ/kg, CP 14%） and low （DE 12.55 MJ/kg, CP 11%） nutritional level,respectively. All pigs were slaughtered when their weight are about 100 kg, and determined PRKAG3 gene expression, carcass characteristics and meat quality traits. The results showed that low nutritional level increased PRKAG3 gene expression （P〉0.05）. High nutritional level increased dressing percentage, lean percentage, loin muscle area, a, b and L values（P〉0.05）, but drip loss was signifieantly lower than that of low nutritional level（P〈0.05）. PRKAG3 gene expression was positive relative to lean percentage, loin muscle area, a value, b value and drip loss（P〉0.05）, hut signifieantly negative relation to pH2 （P〈0.05）. These results suggested that nutritional level influenced PRKAG3 gene expression and so influenced meat quality.