摘要
目的建立白花败酱草中异荭草苷和异牡荆苷含量的高效液相色谱(HPLC)法。方法药材经甲醇超声提取后进行色谱分析,色谱柱为YWGC18柱(150mm×4.6mm,5μm),流动相为乙腈-水-乙酸(22∶78∶1),流速1.0mL/min,检测波长270nm。结果异荭草苷和异牡荆苷分离良好,含量分别在0.012～0.037mg/mL和0.026～0.082mg/mL范围内与峰面积线性关系良好,平均加样回收率分别为96.1%(RSD=0.89%)和98.1%(RSD=1.43%)。结论HPLC法简便、快速,可用于白花败酱草的质量控制。
Objective To establish a method for the determination of isovitexin and isoorientin from Patrinia villosa Juss. Methods The medi- cinat materiats after ultrasound-assisted extraction was analyzed by HPLC with a YWG C18（150 mm ×4.6 mm, 5 μm） column, and the solvent system composed of CH3CN-H20-HAc（22：78：1） was used as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 270 nm. Results An excellent separation was achieved under the optimized conditions, and the linear ranges were 0.012-0.037 mg/mL and 0.026-0. 082 mg/mL for isovitexin and isoorientin, respectively, and the average recovery rates were 96. 1% （RSD =2.06% ） and 96. 7% （RSD =2.24% ）. Conclusion The HPLC method is rapid, fast and suitable for the quality control of Patrinia villosa Juss.
出处
《中国药业》
CAS
2008年第16期24-25,共2页
China Pharmaceuticals
关键词
白花败酱草
异荭草苷
异牡荆苷
含量分析
Patrinia villosa Juss, isovitexin
isoorientin
content determination
