HPLC法测定感冒退烧片中腺苷的含量

Determination of adenosine in ganmao tuishao tablet by HPLC

目的建立HPLC法测定感冒退烧片中腺苷的含量,以便更有效的控制其内在质量。方法采用高效液相色谱法VP-ODS柱（150 mm×4.6 mm,5μm）;磷酸盐缓冲液（pH6.5）-甲醇（17∶3）,检测波长260 nm,流速1.0 ml·min^-1,柱温为室温。结果腺苷在3.93～39.28 mg·L^-1（r=0.9999,n=6）范围内线性关系良好,平均回收率为99.5%,RSD=0.89%。结论本法简便,灵敏,重复性好,准确可靠,可用于感冒退烧片中腺苷的测定。

Aim To establish a method for the determination of adenosine in ganmao tuishao tablet.Methods HPLC method was used.The samples were separated by VP-ODS column（150 mm×4.6 mm,5μm）.The mobile phase was phosphate acid buffer solution（pH 6.5）-methanol（17∶3）.The detection wavelength was 260 nm,and flow rate was 1.0 ml·min^-1.The column temperature was indoor temperature.Results The linear range of adenosine was between 3.93 mg·L^-1 and 39.28 mg·L^-1（r=0.999 9,n=6）.The average recovery was 99.5% and RSD was 0.89%.Conclusion This method is simple,sensitive,accurate and can be used to determine the contents of adenosine in Ganmao Tuishao tablet.