银杏酸、阿奇霉素和大蒜素抗弓形虫增殖效果研究

The anti-Toxoplasma activity of ginkgolic acids,azithromycin and allicin

目的评价银杏酸、阿奇霉素和大蒜素体外和体内抗弓形虫增殖的效果。方法体外试验,培养HFF细胞做为弓形虫宿主细胞,采用MTT法检测3种药物的安全浓度和体外抗弓形虫增殖的效果。体内试验,以腹腔注射的方式给药,观察感染弓形虫小鼠的存活时间,比较3种药物的体内抗弓形虫增殖效果。结果银杏酸和阿奇霉素可显著抑制细胞内弓形虫的增殖,两者效果相近。银杏酸和阿奇霉素均可延长小鼠存活时间。大蒜素抗弓形虫效果微弱。结论银杏酸具有抗弓形虫作用。

To evaluate the anti-Toxoplasma activities of ginkgolic acids, azithromycin and allicin, the human foreskin fibroblast （HFF） cells and KM mice were infected with Toxoplasma gondii RH strain. The MTT assay was used to determine the safe concentrations of the drugs and to evaluate the anti-T, gondii effect in vitro. The effects on anti- Toxo plasma activity of the drugs were also evaluated on counting the mean survive time of the KM mice after intraperitoneal injection. The results indicated that both ginkgolic acids and azithromycin could inhibit the growth of T. gondii in vitro, and the mean survive time of the mice were prolonged with the use of ginkgolic acids and azithromycin in vivo, however, aUicin showed very weak effect on T. gondii in vitro and in vivo. It is evident that ginkgolic acids can inhibit the growth of T. gondii in vitro and in vivo.