正常肝细胞与肝癌细胞内β-葡萄糖醛酸酶免疫电镜定位

Localization of β Glucuronidase in Normal and Malignant Hepatic Cells by Immune Electron Microscope

采用低温包埋、胶体金标记、免疫电镜技术，进行了正常肝细胞和肝癌细胞内β－葡萄糖醛酸酶（β－Ｇｌｕｃｕｒｏｎｉｄａｓｅ，β－Ｇ）的定位研究。结果表明，β－Ｇ存在于内质网，溶酶体和高尔基复合体内，同时观察到肝癌细胞中标记β－Ｇ的金颗粒数目多于正常肝细胞中的数目。对肝细胞癌的早期发现，早期诊断可能具有意义。

he content of β Glucuronidase (β G) is very low in normal human body tissue fragments and body liquid. We studied the localization of β G in normal hepatic cell and hepatic carcinoma by low temperature embedding, immune electron microscope technique. β G was observed in endoplasmic reticulum, lysosome, Golgi complex. We found that the quantity of golden granules labelled β G was more in endoplasmic, lysosome, Golgi complex of carcinoma cell than in normal hepatic cells. It may be of significance for discovering and diagnosing hepatic carcinoma at earlier phase.