摘要
研究了酵母菌催化转化制备天然香料2-苯乙醇的方法。从45株酵母菌中筛选出一株产2-PE的酿酒酵母(Saccha-romyces cerevisiae)HJ,通过单因素和正交设计实验优化其培养基,同时建立了反相高效液相色谱同时测定发酵液中底物和产物方法。采用油酸和PPG-1500原位产物转移发酵技术,2-PE的产量达到2.21 g/L,比单水相发酵摩尔转化率提高了8.6%,部分克服了高浓度2-PE对酵母细胞的毒性作用。
2-Phenylethanol（2-PE）,a natural aroma compound,is prepared by yeast cells presented in this paper. A strain of Saccharomyces cerevisiae HJ,having a high production of 2-PE from L-Phe,is screened out from 45 industrial yeast strains of the lab collection. Through the orthogonal test, the optimum composition of medium is obtained and the RP-HPLC method is established for the separation and determination of L- Phe and 2-PE. The fermentation combined with insitu product removal is applied to the production of 2-PE, and 2.21 g/L of 2-PE can be produced with a conversion rate of 55.5%, which can partly overcome the toxicity of high 2-PE to yeast cells.
出处
《现代化工》
EI
CAS
CSCD
北大核心
2008年第8期38-41,43,共5页
Modern Chemical Industry
基金
国家重点基础研究发展计划("973"项目)(2007CB707802)资助
关键词
2-苯乙醇
酵母菌
生物转化
产物原位转移技术
2-phenylethanol
Saccharomyces cerevisiae
bioconversion
insitu product removal
