避孕套的体外细胞毒性检测实验

In vitro Cytotoxicity Test of Condom

目的:通过两种避孕套的体外细胞毒性实验,探讨适合避孕套的细胞毒性检测方法。方法:按照GB/T16886.5的体外细胞毒性评价方法要求,用琼脂覆盖法和MTT比色法检测两种避孕套的细胞毒性。结果:用琼脂覆盖法检测出两种避孕套的细胞毒性均为2级;用MTT比色法检测时,在浸提液浓度为50%时,相对增值率(RGR)避孕套A为5%,避孕套B为7%,两者差异无统计学意义(P>0.05);在浸提液浓度为10%时,避孕套A的RGR为87%,避孕套B为99%,两者差异无统计学意义(P>0.05);在浸提液浓度为20%时,避孕套A的RGR为42%,避孕套B为77%,两者有统计学差异(P<0.05)。结论:由于MTT比色法具有定量评价的优点,更适合避孕套的细胞毒性检测。

Objective： To probe into the method to test the cytotoxicity of condom by agar overlay test and MTT assay. Methods ： According to the method of GB/T16886.5, the cytotoxicity of two kinds of condom was tested by agar overlay test and MTT assay. Results ： By agar overlay test, the cytotoxicity of two kinds of condom was both 2 grade. By MTT assay, when the extraction was 50%, the relative growth rate （RGR） of two kinds of condom was 5% and 7%, respectively, which showed no significant difference （P 〉 0. 05）, however, when the extraction was 20% , the RGR of two kinds of condom was 42% and 77% , respectively, which showed significant difference （ P 〈 0.05 ）. Conclusion ： MTT assay is suitable to test the cytotoxicity of condom because it is quantitative and more sensitive.