动脉粥样硬化性脑梗死患者载脂蛋白B基因XbaⅠ酶切多态性的研究

THE XbaⅠ POLYMORPHISMS OF THE APOLIPOPROTEIN B GENE IN CHINESE ATHEROSCLEROTIC CEREBRAL INFARCTION PATIENTS

探讨动脉粥样硬化性脑梗死（ACI）与载脂蛋白B基因XbaⅠ酶切多态性的关系。方法：以apoBcDNA片段（LB1．5）为探针，利用Southern印迹杂交技术，检测了56名汉族ACI患者和61名正常对照者的apoB基因XbaⅠ限制性片段长度多态性（RFLP）等位基因频率。结果：发现8．6kb（X^-）和5．0kb（X^+）两条杂交片段，X^+等位基因频率在ACI（0．143）和对照者（0.066）之间差异有显著性（x^2=4．28，P＜0．05）。结论：5．0kb片段可能与ACI有关。

Objective: To explore the relationship between atherosclerotic cerebral infartion (ACI)and Xba Ⅰpolymorphisms of apolipoprotein B (apOB) gene. Methods: Xba Ⅰ allele freqencies of restriction fragment length polymorphisms (RFLP) of the apoB gene in 56 Chinese ACI patients and 61 healthy subjects were examined by using Southern blot hybridization method, with the apoB cDNA probe(LB 1. 5). Results: 8. 6 kb (X-) and 5. 0 kb(X+) hybridization fragments were found. The rare allele(X+) was seen more frequently in patients than in the controls (0. 143 vs 0. 066, X2= 4. 28, p< 0. 05). Conclusion: 5. 0 kb(X+) fragment may affect the onset of ACI.