血管内皮生长因子抗体对肿瘤转移的抑制作用

The effect of antibody against vascular endothelial growth factor (VEGF) on tumor metastasis

目的探讨阻断血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）是否可以抑制肿瘤的转移。方法应用ＩＶＴＡ２ＭＡ－８９１津白Ⅱ小鼠自发乳腺癌模型进行抗肿瘤转移的研究，此模型伴有高自发肺转移。结果Ｎｏｒｔｈｅｒｎ杂交及免疫组化证实，该乳腺癌原发灶及肺转移灶均可表达ＶＥＧＦ，且以后者为高。接种肿瘤后第９天，以ＶＥＧＦ抗体处理荷瘤小鼠，可明显抑制原发肿瘤的生长（４４．０％，Ｐ＜０．０５），而对肺转移灶数目及转移灶大小的抑制率分别达７３．０％和８３．７％。结论ＶＥＧＦ抗体对肿瘤转移的抑制有潜在的应用价值。

Objective To study whether manipulation of angiogenesis with antibody against vascular endothelial growth factor (VEGF) may interrupt the tumor growth and metastasis. Methods IV TA 2MA 891 is a murine spontaneous breast cancer with high rate of metastasis in lung in (TA 2x615) F1 mice, and it was chosen as an animal model in this study because of the high expression of VEGF in primary tumor as well as in lung metastatic tumor. The mice were injected with anti VEGF polyclonal antibody at a dose of 200 μg/mouse/day from day 9 to 23 following sc tumor inoculation and sacrificed on day 26. Results The growth of primary tumor was inhibited by 44.0% and the number and size of metastatic foci in the lungs were inhibited by 73.0% and 83.7% respectively. Conclusion The antibody against human VEGF may be a potentially effective antimetastasis agent for the treatment of patients with lung metastasis.