急性非淋巴细胞性白血病免疫球蛋白基因重排的研究

Study on rearrangements of immunoglobulin genes in acute nonlymphocytic leukemia

目的探讨急性非淋巴细胞性白血病中免疫球蛋白基因重排序列失真现象的机制及其在白血病基因分型中的意义。方法采用多聚酶链反应技术对２５例不同时期急性非淋巴细胞性白血病患者进行了研究。结果２５例患者中，有３例检测出免疫球蛋白重链基因重排，其中１例为完全缓解期患者，观察发现其在检测后１个月内有复发倾向。结论急性非淋巴细胞性白血病中存在免疫球蛋白基因重排序列失真现象。因此，免疫球蛋白基因重排可能并不能作为急性淋巴细胞性白血病诊断绝对可靠的标志。免疫球蛋白基因重排在白血病微小残留疾病检测中有一定意义。

Objective To explore the mechanism of infidelity of immunoglobulin gene rearrangements in acute nonlymphocytic leukemia and its significance in genotyping of acute leukmia. Methods Immunoglobulin gene rearrangement was studied in 25 patients with acute nonlymphocytic leukemia in various stages by polymerase chain reaction. Results Immunoglobulin heavy chain gene rearrangements were detected in three of 25 patients, one of whom in complete remission was found to have tendency of relapse one month later. Conclusion Lineage infidelity of immunoglobulin gene rearrangements does exist in acute nonlymphocytic leukemia, therefore, immunogobulin rearrangements probably can not be regarded as an absolutely reliable marker for acute lymphocytic leukemia. Immunoglobulin rearrangements may have some significance in the detection of minimal residual disease of acute leukemia.