淀粉样前体蛋白在痴呆模型大鼠背海马结构内的表达

EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN DORSAL HIPPOCAMPAL FORMATION OF DEMENTIA MODEL IN RAT

研究用AlCl_3灌胃,建立鼠老年性痴呆模型.用免疫细胞化学ABC法,观察了淀粉样前体蛋白在背海马结构的表达.结果显示在痴呆模型鼠背海马结构双侧各部均可见较强的淀粉样前体蛋白表达,且与对照组间有显著性差异.光镜结果,阳性反应产物主要位于神经元核周质和突起及其分支.细胞大小不等,多数淀粉样前体蛋白样免疫反应阳性神经元胞体为梭形和锥形.背海马结构内淀粉样前体蛋白样免疫反应神经元主要分布在CA1、CA2、CA3和CA4区的锥体细胞层、光辉细胞层及齿状回多形细胞层,其中在CA1和CA3区淀粉样前体蛋白样免疫反应神经元数量最多.说明海马CA1、CA3区淀粉样前体蛋白表达的改变与老年性痴呆的发生有密切关系;同时也提示铝的毒性作用可改变淀粉样前体蛋白在脑内的表达水平.

After pretreatment with aluminium chlorid PO, the model of dementia was established. The expression of β-amyloid precursor protein (β-APP) in the dorsal hippocampal formation was observed with ABC immunocytochemical method. The results showed that β-APP overexpression asfound in the bilateral dorsal hippocampal formation, and there were significant differences between the model group and the control group. The sections were examined by light microscopy, the positively reactive products were mostly located in the cell membrane, cytoplasm and processes. The size of APP-LI neurons vary differently. Most of the APP-LI cells were found to be of fusiform or pyramidal shape. The APP-LI neurons wre distributed mainly in pyramidal cells and the oriens layer of CA1, CA2, CAS and CA4 region of polymorphical cell layer of dentate gyrus; the number of APP-LI neuron was the most in CA1 and CAS region. The results suggest that these changes of the expression of B-APP in CAl and CAS region are related to Alzhermer' s disease, and that aluminium can alter the expression of APP level in the brain.