培养环境对枣组培微嫁接的影响

Influence of Culture Environment on in vitro Micrografting of Ziziphus jujuba Mill.

[目的]建立一套高效的枣组培微嫁接体系。[方法]以感染枣疯病的婆枣组培苗(以下简称染病苗)为砧木,以冬枣组培苗茎段为接穗,研究若干环境因子对枣组培微嫁接成活率的影响。[结果]将枣组培微嫁接苗置于蔗糖浓度为50 g/LMS无激素培养基中的成活率较高。适宜的培养基pH值为6。采用不同封口材料控制培养瓶内湿度的研究表明,棉塞和单层封口膜封口可保持瓶内湿度,并有利于微嫁接苗的成活。微嫁接苗在28℃进行培养较为适宜。在适宜条件下枣组培微嫁接的成活率最高,达33.3%。[结论]培养基蔗糖浓度、pH值、培养温度和湿度均会影响枣微嫁接苗成活率。

[Objective] The study aimed to establish a efficient system of micrografting techniques of Ziziphus jujuba Mill.[Method] Using Pozao plantlets infected by witches broom disease as rootstocks and Dongzao plantlets as scions,the influences of culture environment on the micrografting of Ziziphus jujuba Mill were studied.[Result]The higher survival rate was obtained with the optimum culture medium： MS medium with 50 g/L sugar without plant growth regulations.The optimum pH of medium was 6.Meanwhile,culture bottles sealed with cotton plug and single closure could keep humidity of the inner bottles and was benefit for micrografting.The optimum culture temperature was 28 ℃.At above condition,the highest survival rate of micrografting jujube was 33.3%.[Conclusion] The sugar concentration,pH of medium,temperature and humidity all could affect the survival rate of micrografting.