一种鉴定菜用枸杞的新方法——nrDNA ITS测序法

An New Method of Identification on Edible Lycium Linn.Germplasm Resources——nrDNA ITS Sequencing

[目的]拟从分子水平对菜用枸杞进行鉴定。[方法]利用nrDNA ITS（核糖体DNA基因内转录间隔区）碱基序列测定的方法对5份菜用枸杞资源进行碱基序列测定并分析序列差异。[结果]首次获得5种菜用枸杞nrDNA ITS区碱基序列,整个ITS序列长度变异范围为628～632 bp,平均为630 bp,共有79个变异位点,占12.5%,保守位点553,占87.5%。基于nrDNA ITS序列差异的品种聚结果与依据形态指标的是划分结果相符。[结论]nrDNA ITS区测序分析可作为分子水平鉴定菜用枸杞不同种质来源的又一途径。

[Objective] The study aimed to identify Lycium Linn.at molecular level.[Method] The nrDNA ITS sequence of 5 edible Lycium Linn.germplasm resources were investigated.[Result] The nr DNA ITS regions of five edible Lycium Linn.germplasm resources were sequenced.The whole sequences varied from 628 bp to 632 bp,with the average length of 630 bp.Total 79 variation sites were observed in the sequences,which accounts for 12.5%.[Conclusion] Sequence analysis based on nrDNA sequencing provides a new approach to identify edible Lycium Linn.germplasm resources.