辣椒疫霉菌保存及游动孢子诱导技术研究

Methods of Zoospore Inducing and Preservation for Phytophthora capsici

[目的]探索辣椒疫霉菌保存和游动孢子诱导技术。[方法]辣椒疫霉菌在5种不同培养基中,26℃暗培养3 d,再光照培养6 d后,将培养基平分6块,置于2个培养皿中,加入40 ml无菌水,一个计算孢子囊数,另一个于16℃光照0.5 h后,计算游动孢子数。[结果]辣椒疫霉菌在5种培养基中的菌落形态差异明显,生长速度关系是OMA>CMA>CA>PSA>BA,产生孢子囊数量最多的培养基是BA和PSA,最少的为OMA;释放游动孢子量在CMA上最多,BA、PSA、CA和OMA上无显著差异;该菌在试管斜面中,于16℃下可以保存9个月。[结论]诱导辣椒疫霉菌游动孢子的最佳方法是:将该菌在CMA培养基上,于26℃暗培养3 d,再持续光照培养6 d后,加无菌水置16℃光照0.5 h;最佳的保存温度是16℃;游动孢子的浓度与孢子囊的浓度没有正相关性。

[objective] Researches on methods of zoospore inducing and preservation for Phytophthora capsic.were carried out.[methed]The strain was cultured in five different media for 3 d with darkness,then for 6 d with illumination at 26 ℃.Every medium was divideded into 2 group adding 40 ml sterile water respectivetly.One was used to calculate sporangium yield,the other was lighted 0.5 h at 16 ℃,then zoospores were calculated.[Result] Medium types affected P.capsici colony shape.The colony grow speed was OMA〉CMA〉CA〉PSA〉BA.There were the most sporangia in BA and PSA medium and the least in CMA medium.But the most zoospore yeild in CMA medium.There were no significant difference in BA、PSA、CA and OMA medium.The strain can be preserved for 9 months in test-tube.[Conclusion] It is the best method for inducing zoospore that the strain was cultured in CMA medium for 3 d with darkness,then for 6 d with illumination at 26 ℃.Optimum preservation temperature is 16 ℃;There was no correlation between zoospore and sporangi um yeild in different medium.