不同钠盐浸种对NaCl胁迫下萝卜种子萌发的影响

Effect of Different Sodium Salt Soaking on Seed Germination of Raphanus sativus L. under NaCl Stress

[目的]为农作物的耐盐育种和栽培提供试验依据。[方法]通过不同钠盐浸种和NaCl溶液胁迫萌发试验,找出萝卜(Raphanus sativus L)种子抗盐的有效方法和耐盐程度。分别用浓度为200mmol/L的NaCl、NaHCO3、混合盐(NaHCO3,NaCl)3种溶液浸种,然后配置4种浓度的NaCl胁迫溶液:40、80、120、160mmol/L,分别对萝卜种子的萌发进行盐胁迫。[结果]混合盐浸种明显提高萝卜种子发芽指数和活力指数,促进幼苗生长。NaHCO3浸种明显抑制种子萌发,但明显促进侧根数目增多并提高了叶绿素的含量。促进种子萌发的影响因子顺序是:混合盐浸种>NaCl浸种>对照组>NaHCO3浸种。不同浸种方法处理,均表现出随NaCl胁迫溶液浓度升高,抑制种子萌发,而对幼苗生长的影响在NaCl胁迫溶液浓度较低时,随NaCl胁迫溶液浓度升高促进幼苗生长。相反,在NaCl胁迫溶液浓度较高时,随NaCl胁迫溶液浓度升高,抑制幼苗生长。[结论]混合盐浸种促进种子萌发和幼苗的生长。

[Objective]The aim of the research was to provide references for salt tolerant breeding and cultivation of crops.[Method] The effective method and extent of salt resistance of radish （Raphanus sativus L.） seed was investigated by trial using different sodium salt soaking seeds under NaCl stress. 200 mmol/L NaCl,NaHCO3 and mixed salt （NaHCO3,NaCl） solutions were used in seeds presoaking, then 4 kinds of NaCl solutions with concentrations equally spread from 40 to 160 mmol/L were used in seed germination of radish under NaCl stress. Effect of different sodium salt soaking seeds on seed germination of radish under NaCl stress was analysized through determination of germination percentage, germinative force, vitality index, germination index, lamina fresh weight, lateral root number and chlorophyll content of seedlings.[Result] Mixed salt soaking improved the germination index and vitality index of radish seed, and stimulated the seeding growth significantly. NaHCO3 soaking seed inhibited germination, but improved the number of lateral root and chlorophyll content. The effect of stimulate germination was as follow,mixed salt soaking seed〉NaCl soaking seed〉control group〉NaHCO3 soaking seed. Under different soaking seed treatment,with the increase of the concentration of NaCl stress solution, the germination was inhibited, while the seeding growth increased at low concentration of NaCl with the increase of the concentration of NaCl stress solution. On contrast, the seeding growth was inhibited with the increase of the concentration of NaCl stress solution when the NaCl concentration was high.[Conclusion] Mixed salt soaking could promote seed germination and seedling growth.