决明子叶愈伤组织中大黄素与大黄酚含量的测定

Determination of Emodin and Chrysophanol Contents in Callus of Cassia tora L.Leaves

[目的]通过愈伤组织的诱导培养,提高决明材料中大黄素或大黄酚的含量。[方法]用MS培养基萌发决明幼苗、诱导子叶愈伤组织,用HPLC法测定子叶愈伤组织中大黄素、大黄酚的含量。[结果]子叶愈伤组织中大黄素含量为0.099%,大黄酚含量为0.132%;决明子中的大黄素含量为0.029%,大黄酚含量为0.190%。[结论]诱导子叶愈伤组织的方法有助于提高决明材料中的大黄素含量。

[Objective] Through inductive culture, emodin and chrysophanol contents in cassia seed were increased.[Method]MS culture medium was used to bourgeon seedlings of cassia seed and induce cotyledon callus of cassia seed while HPLC method was adopted to determine emodin and chrysophanol contents.[Result] Emodin and chrysophanol contents in cotyledon callus were 0.099% and 0.312% respectively while they were 0.029% and 0.190% respectively in cassia seed.[Conclusion]The method of inducing cotyledon callus were helpful for increasing emodin content in cassia seed.