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HAART治疗前后HIV-1细胞嗜性分析 被引量：1

Analysis of HIV-1 cell tropism in patients receiving HAART
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摘要 目的分析7例患者在高效抗逆转录病毒治疗(HAART)前和治疗后,艾滋病病毒Ⅰ型(HIV-1)细胞嗜性的改变及影响因素。方法采用BD公司的流式细胞仪,通过绝对计数法测定CD4+T淋巴细胞计数和CD8+T淋巴细胞计数,荧光标记物为BD TriTEST CD3FITC/CD4PE/CD45PerCP。采用核酸序列扩增实验(NASBA)测定病毒载量(生物梅里埃公司提供仪器和试剂)。套式聚合酶链反应(Nested-PCR)对gp120V3环区基因进行扩增、测序分析,并确定HIV-1细胞嗜性。结果在7例患者中,有3例发生细胞嗜性转换,2例是从R5到X4嗜性的转换,1例是X4到R5嗜性的转换。在7例患者中,病毒细胞嗜性转换与患者病毒载量、CD4+、CD8+T淋巴细胞之间无相关性。结论HIV-1细胞嗜性的转换与疾病进展的关系不确定;HAART治疗对嗜性转换的作用还不明确。 Objective To analyze HIV-1 cell tropism in 7 AIDS patients before and after HAART and its related factors. Method Genome DNA was abstracted from patients＇ whole blood and HIV-1 Env gene was amplified using nested- PCR. Then the sequence was analyzed by CEXPRESS,BIOEIDT software. Coreceptor phenotype was predicted after sequencing the V3 loop of the HIV-1 glycoprotein 120 using biosoftware geno2pheno （Coreceptor on website-http：// www.geno2pheno.org）. And HIV-1 cell tropism was finally determined. Result Among the 7 patients switch of HIV- 1 cell tropism was observed in 3 cases,of whom two switched from R5 to X4 and one switched from X4 to R5. The switch of cell tropism had no correlation with patients＇ viral loads, CD4^＋ or CD8^＋ T lymphocytes. Conclusion The relationship between the switch of HIV-1 cell tropism and the progress of AIDS is indefinite, and the effect of HAART on switch of tropism remains unknown.
作者 马素珍 李韩平 鲍作义 李敬云 徐小元
机构地区 北京大学第一医院感染疾病科 军事医学科学院五所八室
出处 《中国艾滋病性病》 CAS 2008年第4期330-332,共3页 Chinese Journal of Aids & STD
基金 国家自然科学基金(No.30771906)
关键词 高效抗逆转录病毒治疗 套式聚合酶链反应 艾滋病病毒Ⅰ型 细胞嗜性 HAART Nested-PCR HIV-1 Cell tropism
分类号 R512.91 [医药卫生—内科学]
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参考文献12

  • 1Pollakis G, Kang S, Kliphuis A, et al. N-linked glycosylation of the HIV type-1 gp120 envelope glycoprotein as a major determinant of CCR5 and CXCR4 eoreeeptorutilization [ J ]. J Biol Chem, 2001,20 ; 276(16) :13433 - 13441.
  • 2Witt A, Kaiser R, Mayer A, et al.HIV colonizing peripheral blood monocytes follow lymphocytic isolates in shifting from NSI to SI genotype[J]. Arch Virol, 1996,141(10) : 1833 - 1846.
  • 3Sing T, Beerenwinkel N, Lengauer T. Learning mixtures of localized rules by maximizing the area under the ROC curve[A]. Hern'andez- Orallo JFC, Lachiche N, Flach P, editors.Proceedings of the ROCAI -2004 international workshop on ROC analysis in artificial intelligence[ C]. 2004,89 - 96.
  • 4Sing T, Beerenwinkel N, Kaiser R, et al. Geno2pheno[coreceptor] : a tool for predcting coreceptor usage from genotype and for monitoring coreceptor-associated sequence alterations [ A] . Proceedings of the third European HIV drug resistance workshop[C]. 2005.
  • 5Fouchier RA, Brouwer M, Broersen SM, et al. Simple determination of human immunodeficiency virus type 1 syncytium-inducing V3 genotype by PCR [ J ]. J Clin Microbiol, 1995,33 : 906 - 911.
  • 6Pastore C, Ramos A, Mosier DE. Intrinsic obstacles to human immunodeficiency virus type 1 coreceptor switching [J ] .J Virol, 2004, 78 (14) :7565 - 7574.
  • 7Shioda T, Levy J A, Cheng-Mayer C, et al. Small amino acid changes in the V3 hypervariable region of gp120 can affect the T cell line and macrophage tropism of human immunodeficiency virus type 1 [J]. Proc Natl Acad Sci USA, 1992,89 : 9434 - 9438.
  • 8De Jong J J, De Ronde A, Keulen W, et al. Minimal requirement for the human immunodeficiency virus type 1 V3 domain to support the syncytium-inducing ( SI ) phenotype [ J ] . J Virol, 1992,66:6777 - 6780.
  • 9Karlsson I, Grivel JC, Chen SS, et al. Differential pathogenesis of primary CCR5-using human immunodeficiency virus type 1 isolates in ex vivo human lymphoid tissue[J] .J Immunol, 2005, 175( 11 ) : 7586 - 7593.
  • 10Foda M,Harada S, Maeda Y.Role of V3 independent domains on a dualtropic human immunodeficiency virus type 1 (HIV-1) envelope gp120 in CCR5 coreceptor utilization and viral infectivity [J]. Microbiol Immunol,2001,45(7) :521 - 530.

同被引文献14

  • 1康辉,王亚男,范霞,刁莹莹,代娣,尚红.淋巴细胞表面CCR5在HCV与HIV感染中表达[J].中国公共卫生,2006,22(4):421-422. 被引量：3
  • 2Wu Z,Liu Z,Detels R.HIV-1 infection in commercial plasma donors in China[J].Lancet,1995,346(8966):61-62.
  • 3Berger EA,Murphy PM,Farber JM.Chemokine receptors as HIV-1 coreceptors:roles in viral entry,tropism,and disease[J].Annu Rev Immunol,1999,17:657-700.
  • 4Schuitemaker H,van't Wout AB,Lusso P.Clinical significance of HIV-1 coreceptor usage[J].J Transl Med,2010,9(Suppl 1):S5.
  • 5Nattermann J,Nischalke HD,Feldmann G,et al.Binding of HCV E2 to CD81 induces RANTES secretion and internalization of CC chemokine receptor 5[J].J Viral Hepat,2004,11(6):519-526.
  • 6Regoes RR,Bonhoeffer S.The HIV coreceptor switch:a population dynamical perspective[J].Trends Microbiol,2005,13(6):269-277.
  • 7Ghany MG,Strader DB,Thomas DL,et al.Diagnosis,management,and treatment of hepatitis C:an update[J].Hepatology,2009,49(4):1335-1374.
  • 8Tamura K,Peterson D,Peterson N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
  • 9Shang H,Zhong P,Liu J,et al.High prevalence and genetic diversity of HCV among HIV-1 infected people from various high-risk groups in China[J].PLoS One,2010,5(5):e10631.
  • 10Zhang L,Chen Z,Cao Y,et al.Molecular characterization of human immunodeficiency virus type 1 and hepatitis C virus in paid blood donors and injection drug users in China[J].J Virol,2004,78(24):13591-13599.

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中国艾滋病性病
2008年 第4期

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