110例尖锐湿疣患者FQ-PCR检测结果分析

An analysis of fluorescence quantitative PCR in detecting patients with condyloma acuminate

目的探讨尖锐湿疣患者人乳头瘤病毒(HPV)感染分型及其意义。方法采用荧光定量PCR技术(FQ-PCR)检测男女尖锐湿疣患者HPV感染分型。结果男性患者44例,检出阳性37例(84.09%),分型结果以HPV6/11型为主(91.89%);女性患者外阴标本33例,检出阳性26例(78.78%),分型结果以HPV6/11型为主(53.84%);宫颈标本33例,检出阳性19例(57.57%),分型结果以HPV6/11型为主(57.89%)。结论HPV6/11型是尖锐湿疣发病的主要型别,荧光定量聚合酶链反应(FQ-PCR)可作为尖锐湿疣诊断的指标。

Objective To investigate the typing, positive rate and significance of human papilloma virus（HPV） from the lesions of the patients with condyloma acuminata（CA）. Methods The typing and positive rate of HPV was detected by fluorescence quantitative polymerase chain reaction（FQ-PCR）. The female patients were also detected by cervix pathological biopsy. Results Thirty seven cases（84.09 % ） were confirmed to be HPV-DNA positive among 44 male patients with CA by FQ-PCR, and the main types of infection was HPV6/11 （91.89 % ）. Twenty six cases （78.78 % ） were confirmed to be HPV-DNA positive in cunnus among 33 female patients with CA by FQ-PCR, while 19 cases（57.57 % ） were positive by cervix biopsy. The main types of infection was HPV6/11 （53.84%）in cunnus and 57.89% in cervix.Conclusion HPV6/11 are the main types causing CA.FQ-PCR can be taken as an index in CA diagnosis.