苯并咪唑对大鼠生精功能和睾丸酶活力的影响

Effects of Benzimidazole on Spermatogenesis Function and Enzymatic Activity in Testis of Rats

目的探讨苯并咪唑对雄性大鼠生精功能和睾丸酶活力的影响。方法将40只清洁级 Wistar 雄性大鼠随机分为低(20mg/kg)、中(100mg/kg)、高(200mg/kg)苯并咪唑剂量组和溶剂对照组,每组10只,各组经口灌胃染毒,灌胃量为0.01 ml/g,对照组给予等量的蒸馏水和吐温80溶液,每天1次,连续80 d。染毒结束后,立即取睾丸和附睾观察其形态,测定各组大鼠体重以及睾丸和附睾重量,并计算睾丸和附睾的脏器系数;测定附睾尾精子数量和精子活动率以及睾丸标志酶的活力。结果对照组和低剂量组大鼠睾丸和附睾呈粉红色,体积大,光滑饱满。高、中剂量组大鼠睾丸和附睾呈紫红色,体积小,萎缩明显。高、中剂量组大鼠睾丸和附睾的脏器系数、精子数量和精子活动率均低于对照组,差异有统计学意义(P<0.01)。高、中剂量组酸性磷酸酶(ACP)和山梨醇脱氢酶(SDH)活力均低于对照组,差异有统计学意义(P<0.05或 P<0.01);且 ACP 和 SDH 活力随着染毒剂量的增加而降低。高剂量组乳酸脱氢酶(LDH)活力低于对照组,差异有统计学意义(P<0.05)。不同组别间葡萄糖-6-磷酸脱氢酶(G-6-PDH)活力比较,差异无统计学意义(P>0.05)。结论苯并咪唑可影响雄性大鼠生精功能,这可能是由于苯并咪唑影响了睾丸组织能量代谢酶导致未成熟生精细胞脱落。

Objective To explore the effects of benzimidazole on spennatogenesis function and enzymatic activity in testis of male rats. Methods 40 male Wistar rats, clean degree, were randomly divided into four groups, 10 in each. The rats in the lowdose group （20 mg/kg）, the moderate-dose group （100 mg/kg） and the high-dose （200 mg/kg） were treated with benzimidazole at different doses by gavage, once a day for 80 consecutive days. Simultaneously, the rats control group were treated with the equivalent volume of distilled water and tween-80. In the end of the experiment, the rats were weighed, the testis and epididymides were immediately excised and weighed, the appearance was observed, and the viscera coefficients of the bilateral testis and epididymides were calculated. The number and motility of sperms in the tail of epididymis, the activity of some enzymes （LDH, ACP, SDH, G-6-PDH） of testis were tested. Results The testis and epididymides in the control group and the low-dose group were pink, large, smooth and plump, but they were mauve, small, obviously atrophic in the moderate-dose group and the high-dose group. The viscera coefficient of the testis and epididymides, the number and motility of sperms in the tail of epididymis were significantly decreased in the moderate-dose group and the high-dose group （P〈0.01）. The activity of ACP and SDH in the moderate-dose group and the high-dose group significantly decreased （P〈0.05, P〈0.01）. The activity of LDH in the high-dose group significantly decreased （P 〈0.05）.There is no significantly dedifference among the activity of G-6-PDH of four groups. Conclusion Benzimldazole can affect the spermatogenesis function of male rats by affecting the activity of energy metabolic enzymes in testicular tissue, leading to the sloughing of immature spermatogenic cells.