不同产区烤烟中主要生物碱含量和组成比例分析

Analysis on Major Alkaloids Contents and Constituent Proportions of Flue-cured Tobacco from Different Tobacco Production Regions

采用气相色谱法对我国4个烤烟主产区(湖南、贵州、云南、山东)烟叶的4种主要生物碱(烟碱、降烟碱、新烟碱、新烟草碱)含量进行了分析测定。结果显示,该测定方法的RSD均小于3.5%,各生物碱的平均回收率在98.6%～103.0%之间,说明该法测定结果准确、可靠;4种主要生物碱含量水平排序为:烟碱>降烟碱>新烟草碱>新烟碱;4个产烟区总生物碱平均含量排序为湖南>贵州>云南>山东;产区烟叶中总生物碱含量与栽培品种关系密切。对检测数据进行统计分析,结果发现4个产区烟叶在主要生物碱及总生物碱含量上存在极显著差异(降烟碱除外),新烟碱、新烟草碱这2种主要生物碱占总生物碱的比例也存在极显著差异。

By using gas chromatography, the authors measured and analyzed the contents of four major alkaloids, including nicotine, nornicotine, anabasine, anatabine in flue-cured tobacco from Hunan, Guizhou, Yunnan and Shandong Province. Results showed that the relative standard deviations were below 3.5% and the average recovery of alkaloids were 98.6% - 103.0%. The content of alkaloids from high to low was nicotine 〉 nomicotine 〉 anatabine 〉 anabasine, and the average level of total alkaloid in four production regions was in the sequence： Hunan 〉 Guizhou 〉 Yunnan 〉 Shandong, which was also closely related with cultivars. Based on the statistical analysis of data, leaves from the four major production regions were significantly different in major alkaloids and total alkaloid at 0.01 level （except for nornictine）, which also held true in the proportions of anabasine and anatabine in total alkaloid.