微乳液中阳离子型Gemini表面活性剂／牛血清蛋白相互作用

Interactions Between Cationic Gemini Surfactant and Bovine Serum Albumin in Microemulsion

用内源荧光法和动态光散射技术研究了不同实验条件下W/O型微乳液中阳离子偶联表面活性剂(C12H25(CH3)2N-(CH2)s-N(CH3)2C12H25.2Br-)(12-s-12,2Br-,s=3、4、6)与牛血清蛋白的相互作用。实验结果显示:微乳液体系中含水量、偶联表面活性剂的含量、牛血清蛋白的浓度、联接基团的长度以及外加盐等因素对表面活性剂/牛血清蛋白的相互作用都有较大的影响。体系中含水量增加,微乳液的最大荧光波长蓝移减小;偶联表面活性剂的含量影响了微乳液的最大荧光波长蓝移;牛血清蛋白浓度较高时,两者之间的相互作用较强;联接基团越长,两者之间相互作用越强,牛血清蛋白越易处于疏水环境中;而盐对偶联表面活性剂/牛血清蛋白静电相互作用有一定的屏蔽效应。

The interactions between bovine serum albumin （BSA） and cationic gemini surfactant alkanediyl-α, ω-bis（dimethyldodecyl-ammonium bromide） （12-s-12, s=3, 4, 6） in microemulsion were investigated by using fluorescence spectrometry and dynamic lighting scattering techniques. When water content is increased, the blue shift of the maximum emission peak of microemulsion becomes small. Gemini surfactant concentration would influence the blue shift of the maximum emission peak. Besides, the interactions between BSA and Gemini becomes stronger as the concentration of BSA is increased. BSA is more likely to locate at a hydrophobic environment as the spacer is longer and the interaction between BSA and Gemini becomes stronger. The electrostatic interation is shield to a certain extent by adding salt.