离体动物细胞对病原菌的免疫防御作用

Immune defense to pathogen using cultured animal cells

目的利用诱导的离体动物细胞系兔肾细胞RK13和昆虫细胞Tn-5B1产生抗菌活性肽类物质,筛选具有抑菌作用的抗菌药物。方法利用热灭活的大肠杆菌DH5α或柠檬色葡萄球菌分别诱导RK13和Tn-5B1,对16 h后的细胞诱导产物应用滤纸片法和双层平板法做抑菌活性检测。结果诱导的细胞系随着诱导时间延长出现了一系列形态变化。诱导产物均对受测细菌金黄色葡萄球菌形成了较明显的抑菌圈,且热灭活的大肠杆菌DH5α诱导RK13的诱导产物较之后者的抑菌圈更明显。结论RK13和Tn-5B1经热灭活的大肠杆菌和柠檬色葡萄球菌分别诱导后分泌了具有抗菌活性的物质。

Objective Cultured RK13 and Tn-5B1 animal cell lines were induced with heat-killed Esherichia coli DH5α or Staphy- lococcus sarophyticus. Methods Analysis by bacterial growth inhibition experiments and two-layer plate method indicated that they generated obvious inhibition zones compare to control. Results Morphological change was displayed on cell lines induced. Staphylococcus aureas to be detected were susceptible to the antimicrobial substance from inducible animal cells in culture. In comparison, the antimi- crobial substance induced with heat-killed Esherichia coli DH5α displayed more obvious inhibition zones. Conclusion Antibacterial substances were produced and secreted into media from induced animal cell lines.