HPLC法测定不同产地甘草中甘草酸的含量

Determination of the contents of glycyrrhizic acid in licorice (glycyrrhiza) from different regions by HPLC

目的用HPLC法测定甘草中甘草酸的含量。方法以C18柱（4．6mm×15cm,5μm）为分析柱，乙腈-2％醋酸溶液（36：64）为流动相；流速为0．6ml／min，柱温25℃，紫外检测波长为250nm，采用外表法定量测定。结果表明甘草酸在0．1630～0．8149μg范围内呈良好的线性关系（r=0．9999）。其平均加样回收率为99．76％，RSD为0．96％（n=5）。结论本方法简便可靠，重现性好，可作为甘草酸的质量控制方法。

Objective To set up a HPLC method for the determination of the glyeyrrhizie acid in licorice from different regions. Methods A C18 column（4. 6mm× 15 cm,5μm ） was used with the mobile phase consisting of acetonitrile-2% acetic acid （ 36： 64）. The flow rate was 0. 6 ml/min, the temperature of column was 25℃, and the detection wavelength was at 250nm. To determinate the quantitative assay using external satadard method. Results The linear of glycyrrhizic acid was good in the range of 0. 1630 - 0. 8149 μg （ r = 0. 9999 ） with the average recovery 99. 76% and RSD 0. 96% （ n = 5 ）. Conclusion This method was simple, convenient, and was appropriate to control the quality of licorice.