摘要
以β_2-微管蛋白基因为定点整合位点,通过原生质体法将多菌灵抗性基因转化到哈茨木霉中,获得了具有多菌灵抗性的生物防治工程菌株.多菌灵抑制抗性转化子菌丝生长的EC_(50)值达471.26μg/mL,比哈茨木霉原菌株提高1 200倍以上;转化子对多菌灵的抗性具有遗传稳定性,且在无选择压力下菌丝生长速度及菌落形态与原菌株无显著差别;抑菌活性检测结果表明,3个转化子对立枯丝核菌均具有较强的抑菌活性,对菌丝生长的抑制率分别为87.5%、86.3%和85%.
In order to construct biocontrol strain with crassa was specifically integrated into β2 tubulin site carbendazim resistance, BenR gene from Neurospora of Trichoderma harzianum using protoplast transformation method. The effective concentrations were up to 471. 26μg/mL for transformations mycelial growth, which appear 1 200-fold higher than those of original strain. All transformants displayed steady resistance to carbendazim, and no obvious difference in mycelial growth and colony characteristics were observed between transformants and original strain. The antagonism test indicated that all transfomants displayed strong vitro antagonistic abilities against plant pathogen Rhizoctonia solani. The inhibitory rate of three resistant transformants against R. solani were 87.5%,86.3% and 85% respectively.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第4期58-62,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家高技术研究发展计划“863”资助项目(2003AA241140)
黑龙江省自然科学基金重点项目ZJN03-04
关键词
哈茨木霉
BenR基因
定点转化
生物防治
Trichoderma harzianum
BenR gene
site-specific integration
biocontrol
