PCNA在前列腺癌及良性前列腺增生症组织中的表达及其临床意义

Expression of PCNA in prostatic carcinoma and BPH tissue and its clinical value

目的探讨PCNA在前列腺癌及良性前列腺增生症组织中的表达及其在诊断前列腺癌的临床意义。方法运用实时荧光定量PCR方法检测40例前列腺癌病理组织以及良性前列腺增生症组织的PCNA的基因表达水平。结果PCNA在前列腺癌组织中表达值为9.23±0.55,在良性前列腺增生症组织中表达值为1.23±0.04,前列腺癌中PCNA表达值高于良性前列腺增生症组织,差异有显著性(P<0.01)。结论前列腺癌组织中PCNA基因呈现为高表达,它有助于对前列腺癌的诊断。

Objective To study the PCNA mRNA expression in prostatic carcinoma（PCa） and BPH tissue by real-time fluorescence quantitative PCR and its clinical value for diagnosing cancer of prostate. Methods A real-time quantitative PCR assay was used to detect the level of PCNA gene expressions in 40 cases with cancer of prostate and 45 cases with benign prostate hyperplasia tissue. Results The expression level of PCNA in PCa and BPH tissue were 9.23±0.55, and 1.23±0.04 respectively and there were significant difference between two groups （P〈0.01）. Conclusion In the tissue of cancer of prostate,PCNA gene shows a high expression. It would contribute to diagnose the cancer of prostate.