交联玻璃酸钠膜应用于肌腱粘连的动物实验研究

Research on the Effect of Membrane of Interlaced Sodium Hyaluronate on Preventing Peritendon Adhesion

采用酰肼交联玻璃酸钠膜作为屏障药物,通过动物实验研究其预防肌腱粘连的作用。将24只新西兰白兔随机分为A、B、C组,选兔子双下肢比目鱼肌腱外侧头制作实验模型,采用自身对照的方法,造成挤压挫裂切割伤止血后,A、B、C组右侧注射等量生理盐水1ml,左侧用交联玻璃酸钠膜包裹肌腱。于术后不同时间切取标本,进行大体观察、光镜下组织评分、天狼星红苦味酸染色偏振光显微镜下观察及电镜观察等分析,比较实验侧与对照侧的差异。结果表明:实验组肌腱的修复粘连明显优于对照组。本实验取得了预期的效果,证实了酰肼交联玻璃酸钠膜生物相容性好,在体内存留时间长,能够产生比较理想的防粘连作用。

To explore the effect of membrane of interlaced sodium hyaluronate on preventing peritendon adhesion with animal experiment, 24 rabbits were divided randomly into group A and B and C, the lateral tendon of rabbit＇s soleus muscle were traumatized with ameliorated Rothkopf and Perterson methods and adopted a self- control design to research . After wrapping the left injured tendon with membrane while treating the other side with saline water, the effect of the membrane on preventing adhesion by various methods and experimental apparatus such as pathology observation, sirius red and picric special dying and electronic microscope were observed when the rabbits were put to death according to experimental schedule. Results showed that the indexes of experimental sides were significantly better than that of the control sides. It＇s concluded that the effect of the membrane of interlaced sodium hyaluronate on preventing peritendon adhesion is quite dependable as we supposed beforehand. For the merits of good biological compatibility and endurable persistence in body, this newly developed material can be used in clinic.