壳聚糖水凝胶的研制及其与C2C12细胞粘附、支持作用的实验研究

Development of Chitosan Hydrogel and the Effect of C2C12 Adhesion and Support

研制可注射的壳聚糖水凝胶材料,并对该材料的理化特性进行检测,同时检验其是否能粘附并支持C2C12细胞生长。对不同温度下的壳聚糖溶液进行pH值的测定,并以壳聚糖溶液和β-甘油磷酸钠溶液为原料,制备温敏性壳聚糖水凝胶,对该材料的粘度及37℃成胶时间进行测定,显微镜下观察水凝胶材料的结构,通过C2C12细胞在其表面上的粘附与生长情况,判定该材料是否具有良好的细胞相容性。结果表明:该材料具有温度敏感性,即室温下为液态,37℃变为固态。当壳聚糖溶液浓度为2%时,加入45%β-甘油磷酸钠溶液后的成胶作用时间最短,约为15min。C2C12细胞在壳聚糖水凝胶材料上的粘附与生长情况良好。壳聚糖水凝胶可作为可注射性组织工程化心肌的支架材料,用于携带有效细胞进行缺血性心脏病的治疗。

To study the material of injectable chitosan hydrogel ,to assess its physico- chemical property and ability for C2C12 to adhere and grow. The temperature - responsive chitosan hydrogel was prepared with β - sodium glycerophosphate and chitosan of which the pH was determined under different temperature and the plastic viscosity and the time of solidification at 37℃ of the chitosan hydrogel were also assessed. Then, C2C12 were seeded on the surface of the material and observed under microscope to determine whether the material had a good cytocompatibility. Results showed that the temperature - responsive chitosan hydrogel was prepared successfully, it was liquid at room temperature while it became solid hydrogel at 37 ℃. The plastic viscosity raised with the time and the solidification time was the shortest （within 15 min） when the chitosan hydrogel was prepared with 45% β- sodium glycerophosphate and 2% chitosan. C2C12 adhered and grew well on the chitosan hydrogel material. Our study proves that temperature - responsive chitosan hydrogel can be used as an injectable scaffolds carrying effective cells for heart disease treatment .