RUNX3基因在肝细胞癌中的表达及其意义

The expression and significance of RUNX3 gene in hepatocellular carcinoma

目的探讨RUNX3基因及其蛋白在肝细胞癌（HCC）中的表达规律及其临床意义。方法采用免疫组织化学sP法、反转录-聚合酶链反应（RT—PCR）等方法检测23例HCC患者肝癌组织和相应癌旁肝组织中RUNX3基因及其蛋白的表达情况。结果肝癌组织中RUNX3基因及其蛋白的表达明显降低，阳性率分别为26．1％（6／23）和17．4％（4／23），明显低于癌旁肝组织的表达阳性率的91．3％（21/23）和87．0％（20／23），差异均有统计学意义（P均〈0．01）。结论RUNX3基因及其蛋白在肝癌组织中表达明显下调，提示RUNX3功能失活可能与HCC的发生和发展密切相关。

Objective To evaluate the expression and significance of RUNX3 gene and protein in hepatocellular carcinoma. Method Reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry SP method were used to detect the expressions of RUNX3 gene and protein in 23 cases of hepatocellular carcinoma and their adjacent non-cancerous tissue. Results The expression of RUNX3 gene and protein were significantly reduced in hepatocellular carcinoma tissue （26.1% and 17.4 % respectively）, which were prominently lower than those in adjacent non-cancerous tissue： 91.3% （21/23） and 87.0% （20/23）, P 〈 0.01. Conclusion The lower-expression of RUNX3 gene and protein may play an important role in hepatocellular carcinoma.