IL-13受体在白血病Dami细胞中的表达及IL-13对其表达的影响

Expression of IL-13 receptor in leukemia Dami cells and influence of IL-13

目的研究巨核细胞白血病Dami细胞是否表达IL-13受体α1和IL-4受体α以及IL-13对其表达的影响。方法无血清培养Dami细胞,提取Dami细胞总RNA,反转录,形成第1条cDNA链,PCR扩增,琼脂糖凝胶电泳检测,Quantity One软件分析电泳条带光密度,数据进行统计学处理。结果Dami细胞表达IL-13Rα1 mRNA。4hIL-13组Dami细胞IL-13Rα1 mRNA的表达明显低于血清组、混合组和空白对照组(P<0.05)。12hIL-13组IL-13Rα1 mRNA表达与血清组、混合组和空白对照组IL-13Rα1mRNA表达无明显区别(P>0.05)。Dami细胞表达IL-4Rα mRNA,且不受IL-13的影响。结论Dami细胞表达IL-13Rα1 mRNA,IL-13短暂下调Dami细胞IL-13Rα1 mRNA表达。

[Objective] To investigate whether Interleukine-13 receptor alpha 1 （IL-13Rα1） is expressed in Dami eells and to observe IL-13 effects on the expression of IL-13Rα1. [Methods] After Dami cells were cultured in serum-free medium for 20 h, total RNA was extracted from these cells. The first eDNA strand was synthesized by reverse transcription. Amplifications were performed, and then the PCR products were detected by agar gel electrophoresis. Data are analyzed by statistical analysis. [Results] Dami cells express IL-13Rα1 mRNA. The expression of IL-13Rα1 mRNA in Dami cells was lower in IL-13 treatment group （4 h） as compared with the serum treatment group, the mixture treatment group （IL-13 100 μg/L＋10% serum） and the blank control group （P 〈0.05）. But the expression of IL-13Rα1 mRNA in IL-13 treatment group （12 h） was not significantly different as compared with the serum treatment group, the mixture treatment group （IL-13 100 μg/L＋10% serum） and the blank control group （P 〉0.05）. IL-4Rα mRNA was expressed in Dami cells. However, IL-13 did not effect on the expression of IL-4Rα mRNA. [Conclusion] IL-13Rα1 mRNA is expressed in Dami cells. The expression of IL-13Rα1 mRNA is down- regulated by IL-13.