Survivin与肝癌细胞增殖和凋亡相关性及其临床病理意义

Effects of Survivin expression on cell proliferation,apoptosis and related clinicopathological features in hepatocellular carcinoma

目的分析Survivin表达与肝癌细胞增殖和凋亡间的相互关系,探讨其临床病理学意义。方法应用免疫组化(SP)法检测44例肝细胞癌(HCC)组织、25例癌旁相应组织、9例正常肝组织中Survivin、Ki-67蛋白的表达情况,用TUNEL法检测其凋亡细胞,并计算增殖指数(PI)和凋亡指数(AI)。结果HCC组织中Survivin蛋白阳性表达率为68.18%,癌旁组织、正常肝组织中均无表达,3组比较差异有统计学意义(P<0.01)。Survivin表达与脉管内瘤栓(P<0.05)、组织学分级(P<0.05)显著相关,与其他临床病理特征无相关性。HCC标本中Survivin阳性表达病例平均PI值为(35±16)%,阴性表达者平均PI值为(16±10)%,两者比较差异有统计学意义(P<0.05)。HCC标本中Survivin阳性表达病例平均AI值为(3.2±1.5)%,阴性表达者平均AI值(5.2±2.2)%,2组间差异有统计学意义(P<0.05)。结论Survivin在HCC中高表达,与HCC患者预后相关。Survivin通过促进细胞增殖、抑制细胞凋亡的方式共同影响肝癌的生物学特性。

Objective To analyze the relationship between Survivin protein expression and cell proliferation as well as apoptosis in bepatocellular carcinoma（HCC）, and evaluate their clinicopathological significance. Methods The expression of Survivin and Ki-67 protein were examed using immunohistochemlcal method, and cell apoptosis was examed by TUNEL method in HCC tissues of 44 patients, para-cancerous tissues of 25 patients with HCC, and 9 cases of normal liver. Cell proliferation index （PI） and apoptosis index （AI） were calculated subsequently.Results The positive rate of Survivin protein expression was 68.18% in HCC group, while no Survivin expression was found in control groups（ P 〈 0.01）. The expression of Survivin protein in HCC was significantly associated with vein invasion （ P 〈 0.05） and histological differentiation （ P 〈 0.05 ）, but was not correlated with the other clinicopathological features; In HCC tissues, the average PI and the average AI in Survivin expression positive group vs negative group were（35± 16）% vs （16±10）% （ P 〈 0.05）, and （3.2± 1.5）% vs （5.2±2.2）% （ P 〈 0.05）, respectively. ConcIusion Overexpression of Survivin protein is closely related to the prognosis of patients with HCC. The expressions of Survivin might influence the biological property of liver carcinoma cell by promoting cell proliferation and inhibiting apoptosis.