SDZ对大肠癌lovo细胞的影响

The effects of SDZ on lovo cell line

目的观察SDZ体外对大肠癌lovo细胞的影响。方法培养大肠癌lovo细胞,加入不同浓度的SDZ,作用24 h4、8 h7、2 h,用MTT法计算细胞生长抑制率,描绘细胞生长曲线。结果SDZ体外对大肠癌lovo细胞的生长抑制率随作用浓度和作用时间的增加而增大。1 000μg/mL,200μg/mL,40μg/mL,8μg/mL,1.6μg/mL SDZ对大肠癌lovo细胞作用24 h,细胞抑制率分别为45%、43%、37%、29%、24%。结论SDZ体外对大肠癌lovo细胞有生长抑制作用,呈剂量依赖性和时间依赖性。

Objective To observe the effects of SDZ on lovo cell line. Methods The rates of growth inhibiting detected by MTT. Results The rates of growth inhibiting were increased with density of SDZ and action time of SDZ. The lovo cells were cultured with SDZ for 24h, the inhibition-rates were 45% , 43% ,37% ,29% and 24% for densities of 1000 μg/ml,200 μg/ml,40 μg/ml, 8 μg/ml,and 1.6 μg/ml,respectively. Conclusions The effects of growth inhibiting was marked for lovo cell line in vitro, and the effects had dose and time dependence. The mechanism may be apoptosis.