拟南芥生长素受体基因TIR1启动子的克隆及序列分析

Isolation and sequencing analysis of the promotor for an TIR1 gene from arabidopsis auxin receptor

以野生型拟南芥(Arabidopsis Columbia)基因组DNA为模板,通过PCR扩增得到拟南芥生长素受体基因TIR1启动子2008片段,将该片断克隆到PGM-T载体上。序列分析表明,该启动子大小为2008bp,RNA聚合酶识别序列TATA-box,TIR1特异表达和增强序列CAAT-box皆完整,与已报道的序列比较仅有3个核苷酸发生改变,同源性为99.85%。将该启动子与GUS基因融合,构建成表达载体后,在拟南芥和烟草叶片中做瞬时表达,结果分析显示:拟南芥和烟草叶片中均有GUS酶活性存在。

TIR1 （transport inhibitor response l ） is an auxin receptor, TIR1 promotor was amplified from Arabidopsis Columbia genome by polymerase chain reaction and cloned into PGM-T vector. Sequence analysis showed that the cloned fragment contained 2008 nucleotides, and shared a sequence homology of 99. 85% with the reported sequence. TATA-box and CAAT-box were considered to be responsible for the specificity and enhancement of gene expression. Gene construct that contained the GUS gene under the control of TIR1 promoter was introduced into Tobacco leaf. The GUS activity was detected both in Arabidopsis and Tobacco leaf.