多巴胺转运体基因多态性与难治性强迫症的关联分析

Association analysis between dopamine transporter polymorphism and refractory obsessive-compulsive disorder

目的探讨汉族人群中难治性强迫症与非难治性强迫症多巴胺转运体可变数目串联重复区（DATVNTR）基因多态性差异。方法采用聚合酶链反应（PCR）和限制性片断长度多态性（RFLP）技术，检测51例难治性强迫症和59例非难治性强迫症的DAT基因型，并比较2组之间的DAT基因多态性差异。结果难治性强迫症7／10、7／11、8／11、9／9、9／10、10／10、10／11、11／11DATVNTR基因型频率（0．0％、2．0％、0．0％、5．9％、2．0％、74．5％、5．9％、9．8％）和非难治性强迫症组（3．4％、3．4％、1．7％、6．8％、6．8％、59．5％、5．1％、3．4％）差异无显著性（χ^2=6．126，P=0．525），且难治性强迫症7，8，9，10，11DATVNTR等位基因频率（1．0％、0．0％、6．9％、78．4％、13．7％）和非难治性强迫症组（3．4％、0．8％、10．2％、77．1％、8．5％）等位基因频率亦差异无显著性（χ^2=4．349，P=0．364）。结论在汉族人群中，DATVNTR基因多态性与难治性强迫症无明显关联。

Objective To explore the difference of dopamine transporter variable number tandem repeat （DATVNTR） polymorphism between refractory and non-refractory obsessive-compulsive disorder （OCD） in the Han nationality. Methods 51 refractory OCD patients and 59 non-refractory OCD patients were took part in this study. The gene polymorphism of DAT in all subjects was genotyped directly via polymerase chain reaction（PCR） and restriction fragment length polymorphism（RFLP） technique,and to compare the differences of DAT polymor- phism between refractory and non-refractory OCD. Results There were no significant differences between refractory OCD（0.0% ,2.0% ,0.0% ,5.9%, 2.0% ,74. 5% ,5.9% ,9.8% ） and non-refractory OCD（ 3.4% , 3.4% , 1.7% ,6.8% ,6.8% ,59.5% ,5.1% ,3.4% ） in 7/10,7/11,8/11,9/9,9/10,10/10,10/11,11/1 lfrequencies of genotype（χ^2 = 6. 126, P = 0. 525 ） , and no significant differences between refractory OCD （1.0% ,0.0% ,6.9% , 78.4% , 13.7% ） and non-refractory OCD（ 3.4% ,0.8% , 10.2% ,77.1% , 8.5 % ） in 7,8,9,10,11 frequencies of allele （χ^2 = 4.349, P = 0. 364 ） for DAT. Conclusion The results suggested that there were no significant association between DATVNTR gene polymorphism and refractory OCD.