清脑栓对局灶性脑缺血大鼠急性期脑水肿、神经元损伤及神经肽作用机制的研究

Mechanisms of "Qingnao Suppository" in Acting on Acute Cerebral Edema, Neuron Injury and Neuropeptide in Focal Ischemic Rats

目的探讨清脑栓救治中风昏迷的作用机制。方法选取大鼠120只,随机分为假手术、模型、治疗、对照等不同时间点组;除假手术组外,采用动脉线栓法,制备大鼠局灶性脑缺血模型;造模成功即开始经肠给药:对照组和治疗组分别给予安脑丸和清脑栓,假手术组和模型组予相同剂量饮用水灌肠。采集标本后,进行脑组织含水量测定、脑组织细胞形态学观察。另取大鼠100只,分组、造模、给药同上。采集标本后,进行神经肽的放射免疫测定。结果清脑栓能减轻局灶性脑缺血大鼠急性期脑水肿的程度,对自主活动障碍有明显改善作用;能减轻脑组织损伤的程度,有降低血ET-1、升高CGRP的作用,与对照组比较有显著性差异(P<0.05)。结论清脑栓救治中风昏迷的作用机制,与其减轻急性缺血性脑血管病脑水肿,进而降低颅内压、减轻其神经元损伤、降低ET-1、升高CGRP等作用有关。

Objective To study the mechanisms of ＂Qingnao Suppository＂ in rescuing stroke coma. Methods 120 rats were randomized into four groups： sham group, model group, treatment group and control group at different times. Except the sham group, the rats＇ arteries were ligatod to make eerebral isehemia model. After modeling, eontrol group and treatment group were respeetively given ＂Annao Pill＂ and ＂Qingnao Suppository＂, while sham group and model were given same dose of water through intestine. After treatment, the water content and eell morphology of cerebral tissues were observed. Moreover, another 100 rats were grouped, modeled and treated as above; their neuropeptide was assayed. Results ＂Qingnao Suppository＂ eould relieve focal aeute eerebral edema and injury, improve autonomie movement, deerease serum ET-1 and inerease CGRP, with striking differenees in eomparison with eontrol group （P〈 0.05）. Conclusion ＂Qingnao Suppository＂ ean reseue stroke eoma by relieving aeute eerebral edema to reduee intraeerebral pressure, alleviate neuron injury, deerease ET-1 and inerease CGRP.