苦参提取液中黄酮类化合物的抑菌作用

Antimicrobial Activity of the Flavonoids in Sophora flavescens Extraction

为探讨苦参中黄酮类化合物体外抑菌活性,采用乙醇回流法从苦参中提取含黄酮类化合物并用分光光度法在510 nm处测定黄酮含量.同时,采用琼脂扩散法研究了苦参黄酮类化合物对2种细菌和3种真菌的抗菌性能,测定了其最小抑菌浓度.结果表明:浓度为0.556 g/L的苦参黄酮类化合物对金黄色葡萄球菌、大肠杆菌抑菌圈直径分别为11,8 mm,对啤酒酵母菌、黑曲霉和产黄青霉的抑菌圈直径分别为11,9,9 mm;苦参黄酮类化合物对金黄色葡萄球菌、大肠杆菌、啤酒酵母菌、产黄青霉、黑曲霉的最小抑菌浓度分别为5.6×10-3,0.56,0.28,0.56,0.56 g/L.由此得出结论,苦参中黄酮类化合物对细菌和真菌都有抑制作用,对革兰氏阳性菌的作用强于革兰氏阴性菌,对单细胞真菌作用强于丝状真菌.

Flavonoids were extracted from Sophora flavescens by means of ethanol refluxing method, and measured by using of spectrophotometer at 510 nm. The antimicrobial activities of flavonoids against two bacteria and three fungi were surveyed through agar diffusion method, and the minimal inhibitory con- centrations（MICs） were also determined by using fluid dilution method. The results show that at the con- centration of 0. 556 g/L, the diameters of inhibition zone of Sophora flavescens extraction against two kinds of bacteria （Staphylococcus aureus, Escherichia coli） and three kinds of fungi （Aspergillus niger, Penicilliurn chrysogenurn, Saccharomyces cerevisiae） are 11, 8, 11, 9, 9 mm, respectively, and the minimal inhibitory concentrations against them are 5. 6× 10^-3, 0. 56, 0.28, 0. 56, 0. 56 g/L, respectively. Consequently, flavonoids extracted from Sophora flavescens exhibite antimicrobial activity against bacteria and fungi. The antimicrobial activity of flavonoids against Gram-positive bacteria is superior to Gram-negative bacteria, and the activity against filamentous fungus is inferior to single cell fungus.