氟康唑纸片扩散法和E-test法检测酵母菌体外药敏试验相关性研究

The study of invitro susceptibility testing of yeasts: comparison of E-test and disk diffusion methods

目的对比研究酵母菌氟康唑纸片扩散法和E-test法的相关性,以期确立一种简便可靠的酵母菌体外药物敏感试验方法。方法分别应用纸片扩散法和E-test法检测151株临床分离的酵母菌对氟康唑的敏感性,采用BOMIC仪自动读取培养板上的抑菌环直径,记录试验结果并对质控数据进行核实,使用WHONET-5.4软件分析结果并进行比较。结果两种体外药敏试验方法的一致率可达84.7%,极重要误差率为0.7%,重大误差率为2.0%,次要误差率为12.6%;对于光滑假丝酵母菌两种方法的检测结果有较大差异。结论应用WHO-NET-5.4软件分析得出氟康唑纸片扩散法和E-test法对于检测大多数酵母菌具有非常好的一致性,但对于部分菌株需要进一步应用常量肉汤法确定其最低抑菌浓度值。

Objective To study the correlation between E -test method and disk diffusion method for determining fluconazole. Methods Fluconazole disk diffusion and E - test method procedures were used for determing susceptibility of 151 strains isolated from the hospital. The diameter of inhibition zones of test and reference strain were read by BIOMIC System . WHONET - 5.4 software was used for analyzing the results and evaluating the correlation of in - vitro susceptibility tests. The two susceptibility tests were performed u- sing CLSI standard. Results A Comparison of the cumulative fluconazole MICs reported by two methods showed a high correlation, ac- counting for 84.7% of all the isolates. Very major error was observed （0.7%）, major error was observed （ 2.0% ） and minor error （ 12.6% ） was found. The results of Candida glabrata had a few difference by two In vitro susceptibility tests. Conclusion Although disk diffusion method shows the better agreement with the E - test method for almost yeasts, a few of yeasts isolated need the procedure of NCCLS broth macrodilution for determining MICs.