大鼠肝脏隔离灌注大剂量5-氟尿嘧啶的病理学研究

A Pathological Study of Isolated Liver Perfusion with Large Dose 5-Fu in Rats

应用大鼠原位肝脏隔离灌注技术行不同剂量5-氟尿嘧啶（5－Fu）肝脏灌注，观察肝脏的病理形态学变化。结果表明，正常肝组织可耐受≤250mg／kg的5－Fu灌注，剂量为150mg／kg时肝脏超微结构为可逆性改变，此种变化与灌注液无5－Fu组、全肝血流阻断组无明显不同。证明在可允许的剂量范围内行隔离灌注5－Fu并不加重肝组织的缺血再灌注损伤。

Isolated liver perfusion (ILP) in situ in rats using different doses of 5-Fu was performed to observe the pathological changes of the liver. The results showed that normal hepatic tissue could tolerate regional perfusion with not more than 250mg/kg of 5-Fu. The hepatic ultrastructures developed reversible changes in ILP with 150mg/kg of 5-Fu, which had no significant difference as compared with those of ILP without 5-Fu and total hepatic vascular blockage. From the above it can be seen that ILP within 5-Fu of the maximally tolerance is not likely to deteriorate ischemia-reperfusion injuries of hepatic tissues.